Software like calcusyn how to#
Here, we describe how combinations can be designed in vitro and how to analyze them using Calcusyn or Compusyn.
Software like calcusyn software#
To calculate a CI, the computer software Calcusyn can be used, taking the entire shape of the growth inhibition curve into account for calculating whether a combination is synergistic, additive, or antagonistic. Using this method, a combination index (CI) is calculated from drug cytotoxicity or growth inhibition curves. One of the most widely used ways to evaluate whether a combination is effective is the median-drug effect analysis method. Several attempts have been made to quantitatively measure the dose–effect relationship of each drug alone and its combinations and to determine whether a given combination would gain a synergistic effect. one drug decreases the side effects of another drug. However, one should realize that an antagonistic action may be desired when toxicity is concerned, i.e. It is essential to test the potency of a combination, before evaluation in the clinic, to prevent antagonistic actions.
A combination can be studied by combining the two agents in various different ways, such as simultaneous or sequential combination schedules. one of the agents counteracts the actions of the other. The definition of antagonism is that the combination is less effective than the single agents, e.g. The definition of synergism is that the combination is more effective than each agent separately, e.g., one of the agents augments the actions of the second drug. However, the study of patterns of possible metabolic and biological interactions in preclinical models, as well as scheduling, should improve the development of most drug combinations. The large majority of combination regimens are being developed empirically and there are few experimental studies designed to explore thoroughly different drug combinations, using appropriate methods of analysis. The primary aim is a mutual enhancement of the therapeutic effects, while other benefits may include decreased side effects and the delay or prevention of drug resistance. Drug combinations are widely used because multiple drugs affect multiple targets and cell subpopulations. However, it does neither include response-surface modeling features nor the variety of options for a systematic comparison of Bliss independence and Loewe additivity.Most of the current therapies against cancer, and also those against immune diseases or viral infections, consist of empirically designed combination strategies, combining a variety of therapeutic agents. In this respect CalcuSyn has similar features as CombiTool. As already noted above the median-effect approach for the case of mutually exclusive agents is identical to Loewe additivity. It is based on the median-effect approach and can be applied to combination experiments with two and three agents.
For example, CalcuSyn seems to be one of the most widely used programs in this field. Moreover, adopting the response surface methodology is, so far, not very common in combined-action assessment. We only want to repeat that to the best of our knowledge there is no other program which has an option to analyze combination experiments both according to the Loewe additivity and Bliss independence approaches. This paper to compare the various programs available in a systematic and detailed manner.
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